| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e326380177b52bb2d554d994044a303
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a2892d10a55826d52a5da8751b5cc5b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44862b3b6602053f6f116674e2353d4a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7db256b8c10206b45da1a2f8aeaf5f49 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-21 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1018 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| filingDate |
2001-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7129126e874ea6fe68c3ab4fa231233e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d0cf62fd8546739ee2bb9cd4262ef65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1be3f7d4ce00f9026aee138abf51e199 |
| publicationDate |
2002-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0246233-A1 |
| titleOfInvention |
Method for preparing a human monoclonal antibody, fragments thereof or antibodies comprising such fragments, resulting antibodies and use thereof |
| abstract |
The invention concerns a method for preparing human monoclonal antibodies or fragments thereof or antibodies comprising such a fragment, from B lymphocytes. The invention is characterised in that it comprises the following steps: a) isolating B lymphocytes from a sample derived from a human subject; b) differentiating said B lymphocytes in plasma cells with at least a non-specific activating system and at least a cytokine; c) immortalising said plasma cells by cell fusion with a fusion line; d) culturing in vitro the fused cells until hybridomas are obtained; e) purifying the monoclonal antibodies produced by the hybridomas; f) optionally cleaving said antibodies into fragments and purifying one or several said fragments; g) optionally combining one or several fragments obtained at step f) with fragments of other antibodies. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11237165-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7932360-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012006596-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9012371-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7927834-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10385128-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10934571-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-019505-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011020079-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE47770-E
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10337045-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3569700-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9260517-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8338172-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8268756-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10670599-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2006325236-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9758805-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10752929-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7919257-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10329596-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9738701-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11578132-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10605808-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007068758-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9303081-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9139642-B2 |
| priorityDate |
2000-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |