http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0244415-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78587acbfc8d4c9e9ac3baa7021087d7 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 |
filingDate | 2001-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c83e5c7365007367bce3255d5942ebf1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b037e35c84e1292ab2ee1e46cb7d96c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb5bc94070367071199fc2619db6fe4d |
publicationDate | 2002-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-0244415-A1 |
titleOfInvention | Method for screening of dna libraries and generation of recombinant dna constructs |
abstract | A method of DNA library screening includes homologous recombination in E. coli utilizing lambda phage recombination functions. Inserting a positive selection marker such as antibiotic resistance into the target sequence by homologous recombination facilitates isolation of target sequences and requires only about 58-100 base pairs of total homology, thus allowing the use of synthetic fragments of DNA for taargeting. DNA vector is designed for genomic library construction that features a novel genetic selection for inserts, automatic subcloning of isolated genomic clones and the presence of a negative selection marker adjacent to the genomic inserts to facilitate later gene targeting. |
priorityDate | 2000-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 392.