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filingDate 2001-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2002-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-0242287-A1
titleOfInvention Method for chromatographic preparation of tocotrienol
abstract A method for chromatographic preparation of tocotrienols from a liquid raw material having high contents of both tocopherols and tocotrienols, which comprises providing a chromatographic separation apparatus of pseudo mobile layer type having a number of columns being packed with a hydrophobic absorbent for reversed phase liquid chromatography having a less affinity for tocotrienols than that for tocopherols and being connected in an end-free and circulative way, feeding a substantially perfectly oily raw material containing a few tens percents of tocopherols and tocotrienols and an eluent having a composition of ethanol/water = 90/10 to the chromatographic separation apparatus, and separating a resultant liquid fraction enriched in tocotrienols from tocopherols. The method allows the chromatographic preparation of tocotrienols on a commercial scale with operations similar to those used in a conventional chromatographic separation method of pseudo mobile layer type, which leads to the reduction of the initial equipment cost and operation cost for the preparation of tocotrienols.
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