| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12a9e9ce63eb0bbf3658453fb50a6d9b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_38a66dad9cdcb481e0a9f683c7c1be50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c07dcb89e7b58897b56b5c01e46efa6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4eac8f7cfce6bc2928b8ca88587a5a61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9a17a5ea87b58910e0a05c7ed078e2f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate |
2001-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfd1fa4ad1075f52aa955afdf0aa152c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5549ba55935f73f589b8eb7910e74349
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb822cd55113cb1944f539d957435497
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8dad8987abc4c53a4dab038a195e1044 |
| publicationDate |
2002-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0229067-A1 |
| titleOfInvention |
EXPRESSION VECTORS WITH MODIFIED ColE1 ORIGIN OF REPLICATION FOR CONTROL OF PLASMID COPY NUMBER |
| abstract |
In an expression vector having a ColE1 replication system, the homology of the RNAI and RNAII of the ColE1 origin of replication to uncharged tRNAs is modified mutations in the coding region of the RNAI gene and corresponding mutations in the RNAII gene. The mutation results in one or more base exchanges in loop 1 and/or loop 2 and/or loop 3 of RNAI and RNAII. In methods using this vector for producing recombinant proteins, plasmid copy number is stably maintained. In methods for plasmid production, high plasmid copy numbers can be obtained. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011007005-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021255165-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011007005-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101023066-B1 |
| priorityDate |
2000-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |