| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04acc1e0bb9d328afadd66325792bb08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_63039bb0841250d40c7a0b7d99b70f7e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e6e046abd9dda6ec6561a44555b5993a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ed7e93ca40715d49b8647f455da73ec |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-05
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-70 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-076
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate |
2001-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29770d1c85128823e38be7e64be7b48c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_193ee5cee8918016496fb1d361593141
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c96312ce330950fd8eb1260734283fd7 |
| publicationDate |
2002-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0224872-A2 |
| titleOfInvention |
Manipulation of spermatozoa |
| abstract |
The present invention relates to a method of sperm permeabilisation comprising: (a) washing a sample containing sperm in a sperm nuclear extraction buffer (SNEB) which includes one or more metal chelating agents and which is substantially free of non-ionic detergents and divalent cations; (b) contacting the sperm with one or more permeabilising agents; and (c) optionally separating the sperm tail from the sperm head; and to a method of sperm transformation which method comprises incubating a sample of sperm or sperm nuclei in one or more cell-free extracts. Also described are extracts and buffers for use in these methods, methods of preparing the extracts and uses of the transformed sperm nuclei. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7125976-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7736895-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1356277-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1356277-A4 |
| priorityDate |
2000-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |