| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb141b396e502bfce986c491d722e401
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5c2c7e7500325e34709fd559eafd7e50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8ac709777109bc769759ebaaa8be15e0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_40a442dab38f6df983c8a7e3cddd3357 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2002-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d73b74ddf6d28082adc6753a48179ada
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eab2c7a8df9b2a197003be6f18ff1119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a91f5690431388f0f5c3b2d92ba61a6 |
| publicationDate |
2002-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-02095002-A2 |
| titleOfInvention |
N4 virion single-stranded dna dependent rna polymerase |
| abstract |
A histidine-tagged, deletion mutant of bacteriophage N4-coded, virion RNA polymerase (mini-vRNAP) which is active has been developed. The his-tagged mini-vRNAP has been cloned under the control of the Pbad promoter, is stable and is purified in a single step yielding large amounts (10 mg/liter of E. coli expressing cells). This RNA polymerase uses single-stranded DNA containing 17 bases (the promoter) upstream of the transcribed regions as a template. In the presence of E. coli SSB protein, it transcribes this template efficiently, providing a unique system to synthesize RNAs of the desired sequence using single-stranded DNA templates. The enzyme incorporates derivatized nucleoside triphosphates with high efficiency. A mutant of mini-vRNAP has been generated that incorporates deoxynucleoside triphosphates. In addition, the inventors have developed an in vivo system to express RNAs and proteins under mini vRNA polymerase promoter control. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004048594-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7838270-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004048594-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3947663-A4 |
| priorityDate |
2001-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |