http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02094998-A9
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_94d54b4c8bff47b482fb887bbd9abdde http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e40d73555b988e4cb5b6af42895d8aab http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fedb3f99b5dbfa1d7cca4b65f5210d3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_98fad690cf69a4f167f5c5222a015119 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-808 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2002-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a7cd6b02e9daac43c7c00cb54f3cdda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d96ca549583aae99e06cd7544a02b32 |
| publicationDate | 2003-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-02094998-A9 |
| titleOfInvention | Analyzing phosphorylated proteins |
| abstract | Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone phosphorylation. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and an IMAC resin for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of phosphorylation activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in phosphorylation of the target polypeptide. The interaction between the IMAC resin and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE44437-E http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9110075-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9110066-B2 |
| priorityDate | 2001-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 948.