| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70b0b83874bef9ebdf51e1edca2a36be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d1eee7eae576cc58dca3cc04f68f6aef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eef0ce67abe916b5ccacd988d689eeda
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_61e69367264aa47694c1f6abe8c1c30c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7ad58d3c0b071069a8ee0cb8c8ba2e8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S128-24 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61J1-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M45-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-20 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61J1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61J1-05 |
| filingDate |
2002-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43d10c1a16b705c56c7cdaba49c3e1c7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d93a13ba096bc84173be3eed264202a9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5da29bbae533174d8dc3718c220f9524
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e592f49ed705176cd63bfc73088c52d |
| publicationDate |
2002-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-02090489-A1 |
| titleOfInvention |
A cryopreservation bag assembly for mammalian cell lines |
| abstract |
The invention is a novel cell freezing and storage bag assembly and method for using the assembly in the seed train expansion of mammalian cells. The bag is constructed principally of polytetrafluoroethylene fabric. The bag is designed to hold enough cells that a bioreactor can be inoculated directly. The bag is designed to be filled to a fraction of its maximum capacity so that the cell suspension has a very thin cross-section (less than about 10 millimeters). The bag design includes a transfer set that can be sterilely welded to the source of the mammalian cells. This sterile-weldable transfer set allows the bags to be filled quickly with minimal risk of contamination. In the method, once each bag is filled, it is sealed below the connection with the transfer set and the bag is cut 'above' the new seal (on the same side of the seal as the transfer set). When a bioreactor is to be inoculated, the contents of the bag are drained via a sterile-weldable inoculation line. During freezing and storage, the inoculation line is protected from mechanical damage. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006069389-A1 |
| priorityDate |
2001-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |