| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6e3aafe41641e5e32db6a9b6e613d927
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3ff2645d283072344c0c7c8ac14235d8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2cd65e9ce37f5b0da056e3deeeb1a2f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bbf57231ed540b2e01128395c7eef96a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_660445c14a699cceec88639ca743e861
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb93ff947eed3db1391b54490a6aada2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_21864c68b8d7f4d404a470f859abfd2c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_105e17c008326a5368b3a0e3e6eaf8a8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0d7fe0da4731a163c6d63344d45ee541
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ea7160a2a922a2cc5155efb7318bcc9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ebe8446843d92527d56c6acbccba399
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca50270be0c1a76b6658cc412274f536 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate |
2002-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4a935ca3fe83a430a32c8935087411d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1f441d4231c269aab7281d7a0025eee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ceb910c65a75e189b2ec4eb2b966989
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f28010ca8346650bbadd9c0f56175db
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bbc11832b8f9be12d0c1d8d32c60f29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3369b7f1321dbc1448c81068b9df8be5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce7b1f8aca33f92f24f08e7dcdcf1929
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7576980847a92799c4f3939fee2174e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ffc4111fc59303c1429e5c515f2b504
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1668291a29a441f35af98225a6eb9299
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd17ffc342f512b817eb809f5429b3cd |
| publicationDate |
2003-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-02086134-A3 |
| titleOfInvention |
Buffer solution for electroporation and a method comprising the use of the same |
| abstract |
The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using an electric current and to a method for introducing biologically active molecules into animal or human cells using an electric current and a buffer solution. The inventive buffer solution has a buffering capacity of at least 20 mmol *l-1*pH-1 and an ionic strength of at least 200 mmol *l-1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25 °C. The use of a buffer solution of this type in the corresponding method allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in said buffer solution. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9213999-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8257941-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8058065-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8129187-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9499797-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8278104-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8211697-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8048999-B2 |
| priorityDate |
2001-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |