| abstract |
A method of purifying a calcium ion-binding protein by the cation exchange method whereby the calcium ion-binding protein is conveniently and efficiently separated and purified from a liquid containing the calcium ion-binding protein and contaminants without resort to any pretreatment of, for example, adding a chelating agent. More particularly, the method comprises, in the process of purifying a calcium ion-binding protein, adsorbing the protein by a cation exchanger in the presence of calcium ion, washing and then eluting. A calcium ion-binding protein obtained by this method which is substantially free from any impurities. |