| abstract |
The present invention is progestation gene alarm diagnosis kit and the detection method thereof, which includes the DNA extraction fluid for extracting the DNA template of subject, PCR mixture for amplification specifical fragment of subject genome DNA, DNA polymerase, PCR products enzymolysised mixture, restriction endonuclease, as well as positive control template of homozygous mutation and the associated tubes of PCR reaction. Said alarm genes include N5, N10-methylenetetrahydrofolate reductase and methionine synthetase reductase genes, their mutation sites are 677 cytosie→thymine and 66 adenine→guanine respectively, their primer sequences are 5'-TGA AGG AGA AGG TGT CTG CGG GA-3',5'-AGG ACG GTG CGG TGA GAG TG-3',5-GCA AAG GCC ATC GCA GAA GAC AT-3',5'GTG AAG ATC TGC AGA AAA TCC ATG TA-3' respectively. It can diagnose at progestation whether the offsprings will occur neural tube deform. |