http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02079516-A1

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_43b6da0b7e905dfc60e7126e7c0db997
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
filingDate 2002-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6133531cbc87c7600e349bf10e530b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77d8245a21ef1971ff3e58a88d1e8b16
publicationDate 2002-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-02079516-A1
titleOfInvention Nucleic acid analysis using non-templated nucleotide addition
abstract One embodiment of the invention is a method of producing an oligonucleotide extended by a single nucleotide base. An oligonucleotide and an extension terminating nucleotide are mixed with an enzyme having terminal transferase activity. The reaction produces an oligonucleotide extended by a single base. The extended oligonucleotide may be used as a size standard for single base extension reactions. Another embodiment of the invention is a method of producing a mixture of oligonucleotides extended by different single bases. An oligonucleotide, a first extension terminating nucleotide, and a second extension terminating nucleotide are mixed with an enzyme having terminal transferase activity. The first and second extension terminating nucleotides comprise different nucleotide bases and are labeled with different labels. The identity of the different extension terminating nucleotides (and hence the extended oligonucleotides) may be ascertained by reference to the specific label incorporated.
priorityDate 2001-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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