http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02070735-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2f20bd949f345d3611d51d63e0a8872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fa450a9bddc3bf0f630bc172df06f3d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_257da51daae0df66b0684ec2d8580b28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8be6a6c990669567cc455d32b00535e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2002-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_313fdc352b845b13a25f1d5666a53cb2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba8e9b9e840a353c7b628e22e81a809b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa64aad8c66b3f08430bd3905f06fec8 |
| publicationDate | 2002-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-02070735-A2 |
| titleOfInvention | Method for the amplification and detection of dna using a transcription based amplification |
| abstract | The present invention is directed to a transcription based amplification method for the amplification of DNA targets starting from ds or ssDNA optionally present in a sample, comprising the steps of: - incubating the sample in an amplification buffer with one or more restriction enzymes capable of cleaving DNA at a selected restriction site, said restriction enzyme creating a defined 3' end on the said DNA strand(s), and a promoter-primer, said promoter-primer having a 5' region comprising the sequence of a promoter recognized by a DNA-dependent RNA polymerase and a 3' region complementary to the defined 3' end of the DNA strand, a second primer, having the opposite polarity of the promoter-primer and comprising the 5' end of the target sequence, and in case of ssDNA as the target DNA, a restriction primer; - maintaining the thus created reaction mixture under the appropriate conditions for a sufficient amount of time for a digestion by the restriction enzyme to take place; - subjecting the sample to a heat treatment at a temperature and time sufficient to inactivate the restricting enzyme and/or to render a double strand single stranded; - adding the following reagents to the sample: an enzyme having RNA dependent DNA polymerase activity, an enzyme having DNA dependent DNA polymerase activity, an enzyme having Rnase H activity, an enzyme having RNA polymerase activity; and - maintaining the thus created reaction mixture under the appropriate conditions for a sufficient amount of time for the amplification to take place. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02072881-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9464308-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005083123-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8206902-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005093095-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02072881-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7723078-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009074315-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009074315-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8293476-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7803579-B2 |
| priorityDate | 2001-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.