http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02065096-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_582d3500df1e8a6086f8edabda2eac68 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2002-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d630cbc449c0975df7a12e2a5f61f7b1 |
| publicationDate | 2002-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-02065096-A1 |
| titleOfInvention | Method for treating histological and biological samples |
| abstract | The inventive method for treating histological and biological samples consists in successive immersing the samples into an aqueous solution of fixing substance, at least into three dehydrating liquids containing water, and at least into three dehydrating liquids devoid of water. A chemical mark is added to the water contained in the aqueous solution of the fixing substance. A chemical indicator is added to the dehydrating liquid which does not contain water. Said indicator together with the chemical mark produce a coloured composition which is substituted when the dehydrating liquid free of water is coloured in such a way that it is visible to the unaided eye. Ethyl and isopropyl alcohol and mixtures based thereon are used as a dehydrating liquid. Salts of transitional metals having the oxidation rate of 2+, such as salts of iron, cobalt, nickel, copper and zinc in a quantity of 2-40 g (in the metal equivalent) per litre of water are used as a chemical mark. Orto-phenanthroline, diphenylthiocarbason, α nitroso-β-naphthol, sodium diethylthiocarbamat, 8-oxyquinoline, 1-(pyridilazo)-2-naphthol, 1-(pyridilazo)-resorcin and 2-carboxy-2'- hydroxy-5'-sulphoformasilbenzol in a quantity of 0.001-04 g per litre of alcohol, or potassium or sodium rodanade in a quantity of 5-10 g per litre of alcohol. |
| priorityDate | 2001-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.