Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a59dbfbd5f9be9294052d971a7ae1fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9cdc0f26f8cace0e345e44e0ebfee8e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e19741e967ed6c909c17813b4f9ef63e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f4eb956d4dfa0740b1904c2cb6740f6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0046dee1ecdb900fb546597fc3941d8 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2001-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_945eb6b92421ca85380e41babdab81f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0029123f84924fd2ea2e2f6e40ac9a02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccb747b71701075be63530b266801fc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f100b0490b727680808b1a25de202c1 |
publicationDate |
2003-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0204666-A3 |
titleOfInvention |
Decondensation of dna |
abstract |
Decondensation of DNA is accomplished by treating DNA-containing cells with a thiol and, simultaneously or subsequently, with anionic surfactant wherein the cells are exposed to the thiol for less than 20 minutes. The cells are preferably sperm cells and the method is particularly useful in livestock sperm such as bovine sperm. Sperm cells may be subjected to fluorescent in situ hybridisation after decondensation. The invention also provides kits comprising thiol and anionic surfactant and treatment instructions. The methods and kits are useful in assessing the efficiency of sperm sorting procedures. |
priorityDate |
2000-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |