| abstract |
The invention relates to a method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A an oligopeptide comprising 3 adjacent amino acids identical to or corresponding to the amino acids of the C-termninal amino acids 1-40 of VirF, VirD2, VirE2, VirE3 or MobA, and ii) as a second part B a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof with the N-terminal end of the first part. |