| abstract |
The present invention provides variants of an immunoglobulin variable domain having at least one CDR region and framework regions flanking the CDR. The variant includes (a) the CDR region having added or substituted therein at least one binding sequence and (b) the flanking framework regions, wherein the binding sequence is heterologous to the CDR and is a binding sequence from a binding site of a binding pair, and wherein the binding sequence is a CD80 and/or CD86 receptor-binding portion of CD28. Also disclosed and claimed are molecules, immunoglobulins, and compositions including these variants, as well as nucleic acids and expression vectors encoding these variants, and methods of using the variants for immunotherapy. |