Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f1982c9b56bd03f0ecfc4f2a9b6ae78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bf0fca338f679506012865aaa6c0f590 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_58450f9efd1064ea2fc59f8cdc2d0a4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_94d9878acf561e9fba58343eee731789 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate |
2001-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c22931e86e1726be047c1c98a144cdd8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99ef21b60e42f8f26d30e550f0a51eed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e01ada839d87dd46d71e48d98383426c |
publicationDate |
2001-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0183823-A1 |
titleOfInvention |
Methods and compositions for polynucleotide analysis using generic capture sequences |
abstract |
Methods, compositions and articles of manufacture for assaying a sample for an amplification product from a target polynucleotide are provided. An amplification reaction is used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay the sample for the target polynucleotide. A sample suspected of containing the target polynucleotide is contacted with first and second primers to amplify the target polynucleotide; the first primer comprises a tag sequence, the complement of which is formed on the opposite strand during amplification and is referred to as a capture sequence. That opposite strand is referred to as a second primer extension product or an amplification product, and comprises a label. A capture probe is provided that is conjugated to a substrate and can bind to the capture sequence to form an amplification product detection complex. Methods of detecting the amplification product thus produced are also provided, as are amplification product assay arrays, along with methods of forming the same. The methods are particularly useful in multiplex settings where a plurality of target polynucleotides are to be assayed. Kits comprising reagents for performing such methods are also provided. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010518862-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2518162-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9453262-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016023430-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110368956-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2126072-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2126072-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005117514-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016097953-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110368956-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008061193-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104178478-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8603749-B2 |
priorityDate |
2000-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |