http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0183802-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_424db9d56b06a23aed410fcf5df652f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d98ee604a6d8717f0b0c75c288cee56 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-006 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 |
filingDate | 2001-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b37b57a05d70b53138c08422991de70b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_814a2c6999fd265b67971360309f8730 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d5592d92f3a90b0b5a0410dc0d88d9c |
publicationDate | 2001-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-0183802-A1 |
titleOfInvention | Whole cell and cell-debris free polysaccharide |
abstract | The present invention provides for a method for obtaining a polysaccharide substantially free from whole bacterial cells and bacterial cell debris including: (a) fermenting a Sphingomonas bacterium, which produces the polysaccharide, to produce an aqueous fermentation broth having the polysaccharide dissolved therein; (b) diluting the fermentation broth with an equal volume of deionized water; (c) partially hydrolyzing the polysaccharide by exposing the fermentation broth resulting from step (b) to a temperature in excess of 100 °C for a time period from about ten minutes to about one hour; (d) removing bacterial cells from the polysaccharide by centrifugation of the fermentation broth and recovery of a supernatant aqueous liquid; (e) precipitating the polysaccharide from the supernatant aqueous liquid by adding a second liquid to the supernatant aqueous liquid, wherein the second liquid is miscible with water, non-reactive with the polysaccharide, and in which the polysaccharide is substantially insoluble; and (f) harvesting the polysaccharide resulting from step (e) by separating it from the supernatant aqueous liquid. |
priorityDate | 2000-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.