| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_662717c94dd93edb84c2df5ba0d5e45c |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2001-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e06ed7b7216d56b6c139656719935a43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13e7c4b6c9bf9838d524c246d2904cb9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69fe0e858f35fa57105c7fdbcc6b1c81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1332303327af778680646b4cc5bb737
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55bd23e374fde4f8377a58b0ab757d36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ead4502947fd9b3fa3af4ce64108aeab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9788b95460cd6079b8a4bf967bb4dfa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04270a95693cdcea6ec4818900632143 |
| publicationDate |
2001-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0181566-A2 |
| titleOfInvention |
Apparatus and method for separating and purifying polynucleotides |
| abstract |
The instant invention provides a non-HPLC chromatographic method for purifying a target polynucleotide comprising the steps of: applying the target polynucleotide to a separation medium having a non-polar separation surface in the presence of a counterion agent, whereby the polynucleotide is bound to the separation medium; eluting the target polynucleotide from the separation medium by passing through the separation medium an elution solution containing a concentration of organic solvent sufficient to elute the target polynucleotide from the separation medium; and collecting the eluted target polynucleotide. The separation medium can be supported in any of a variety of containers, non-limiting preferred examples of which include spin columns and vacuum trays. The invention is particularly useful for the separation of RNA and single and double stranded DNA. In preferred embodiments of the invention the purification is accomplished under conditions that are substantially free of multivalent cations capable of interfering with polynucleotide separations. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9656228-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010004253-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6521411-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10590161-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10118173-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10077439-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2378445-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2007304046-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11377470-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2378445-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10138507-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008040959-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008040959-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2983804-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2009269872-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6812341-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10898899-B2 |
| priorityDate |
2000-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |