http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0175155-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7f00b6ea9ce5ef14516bdce8c69e4775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_00318c9b21f99e6b661ec77dda42ca4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_79db4d4b06d99f0312c72aca6385f63d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_848a8d507001018e287f1f70e2551c47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6e10d2a9afa49d345275857d8711dc5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2001-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1997eb4c53c76a6a6d0f65e6172a3ad8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19d06d871ed09012f9f2e0312cd04da8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18e460b3c53875e3cb406228d397d35d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1486c2c176be4bb0f9f0642f9a033099 |
| publicationDate | 2001-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-0175155-A2 |
| titleOfInvention | Methods for detecting of nucleic acid amplification reaction products |
| abstract | Methods are described that can distinguish between specific and non-specific amplification products and which may be quicker, cheaper and/or more convenient to use than existing methods, particularly in relation to high-throughput applications. Small molecules, for example high-pH buffers, are added to the post-amplification reaction before determining the presence/absence or quantity of double-stranded nucleic acid present. The problem of background fluorescence caused by short but abundant non-specific amplification products and by excess reactants of the amplification reaction is thereby addressed. Identification of the amplicon (specific amplification product) by a single fluorescence reading step, which may readily be automated, is achieved. The methods may be particularly suitable for use with ARMS-PCR methods for SNP typing, in particular HLA typing. The method may be useful for identifying numerous sequence polymorphisms, such as those within the DR locus of HLA Class II. The small molecules may increase the pH in the sample to at least 11 or less than about 5 and/or may destabilise double stranded DNA. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979333-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111979333-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004061130-A1 |
| priorityDate | 2000-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.