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filingDate 2001-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9894bc00b944e4fdb6ec23c44893d7fa
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publicationDate 2002-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-0175072-A3
titleOfInvention Production of TcR gamma delta T cells
abstract The method for obtaining and expanding TcRηδ+ T cells in culture is described. The method involves: (1) culturing cells from a sample containing TcRηδ+ T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two growth factors and (2) culturing the cells obtained in step (1) in a second culture medium comprising at least two growth factors. The two growth factors are factors with interleukin-2-like and interleukin-7-like activity. The TcRηδ+ T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.
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