| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_57a631da4914c9e276b8db4b68a9e7d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_675f16e42f230e209d29fb3b0cb54da8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate |
2001-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af0b7fc244f2931f3ed596518f3b4fc1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91a99bf2edbbc9a99d47d8cca60e0e67 |
| publicationDate |
2001-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0157183-A2 |
| titleOfInvention |
Method for producing circular or multimeric protein species in vivo or in vitro and related methods |
| abstract |
A method is disclosed for the in vitro or in vivo cyclization of protein or peptide sequences. Also disclosed is a method of fusing polypeptide sequences while bound to a solid support. These protein manipulation techniques relied on the trans-splicing activity of a split intein, such as the naturally occuring split intein form the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein). The cyclization procedures required the fusion of C- and N-terminal intein splicing domains to the N- and C-termini, respectively, of a target protein (Inteinc-target protein-InteinN). Cyclization in vivo occurred post-translationally when the two complementary intein splicing domains ligated the N- and C-terminus of the target protein. In vitro cyclization also utilized and InteinC-target protein-InteinN precursor protein, in which the intein domains were fused to a chitin binding domain (CBD). Protein expression was conducted under conditions that favored the accumulation of precursor protein, which was immobilized on a chitin resin. The circular protein species were eluted from the chitin resin following incubation under conditions that favored protein splicing. Trans-splicing was used to ligate polypeptides on a solid support by generating a protein composed of a CBD fused to a C-terminal intein splicing domain and target protein (1). This was incubated with a protein composed of target protein (2) fused to an N-terminal intein splicing domain and a CBD. The precursor proteins were immobilized on a chitin resin where trans-splicing resulted in the ligation of target protein (1) to target protein (2). These techniques greatly expand the procedures available for protein engineering and modification. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6987007-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6984505-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021179860-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7378248-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2078040-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7208571-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7250551-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03066861-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8878004-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2078040-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9888707-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8664476-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9409955-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013033456-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0166565-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6897285-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9474293-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7105341-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0166565-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10006038-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9249474-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9601227-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7566765-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9040462-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7060465-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10428332-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7252952-B2 |
| priorityDate |
2000-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |