Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5e6996299cade2a994fcb12ee9872bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e64551a2a6c381657c58745036d5ef07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f0f7ca13b0795cdc64ad5408c1b879de http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_06151009ee349551a4c629d6ea0ea674 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dea97595058f316a875c28a64efb90da http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7d36d2b49f646d83d05e9b38102d8f2 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-90241 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate |
2000-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_953e09813726dd3b9baf3284e161b11d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fbf2105eb018f02949c8c8b985fb21d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18ca8f3371961e78e9120a238f773495 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2c165006b3a54032872c7b668c7a3bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b1d81c7a68f3c9f5163a00f7c1d223a |
publicationDate |
2002-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0131028-A3 |
titleOfInvention |
Mutant luciferase |
abstract |
A recombinant protein having luciferase activity and at least 60 % similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9139836-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9487520-B2 |
priorityDate |
1999-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |