| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1010e3862914acdd087e73d76911ec6d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f96545cd2ad055a17bc7a1cbb0075178
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9feb10216a71c6b152f703726f0d4960
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ada99e6c9ab0fe32d07aa15b40660892
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0fbfd1c784eeff0003f50867385fc470 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-968
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-975
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-97
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-40 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6863
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2000-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d36d45a7f4116839ef7c1866f0ace3a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bef187e9d89f9eee73f11d151e45119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fce8bd902d4f69b8dfcc19dfb41208a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b88bc594154cf29f6156de768d7db4f3 |
| publicationDate |
2001-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0123891-A1 |
| titleOfInvention |
High sensitive immunoassay |
| abstract |
A time-resolved fluorescence immunoassay (TR-FIA) for detecting cytokine in a liquid biogenic sample, which comprises a step of capturing cytokine and forming its complex with a lanthanoid metal ion having a fluorescent moiety on a solid phase and a step of measuring the fluorescence from the fluorescent moiety, wherein the complex comprises (a) a first antibody having a moiety bound to the solid phase and a region capable of binding with cytokine, (b) cytokine, (c) a second antibody having a region capable of binding with cytokine and a moiety bound with biotin, (d) a connective having streptoavidin or avidin and a fluorescent moiety capable of forming a complex with a lanthanoid metal ion and (e) a lanthanoid metal ion, which are bound sequentially in this order. The flurescent moiety is represented by the general formula (I). The immunoassay can be used for detecting cytokine in a biogenetic liquid sample with improved sensitivity. |
| priorityDate |
1999-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |