| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b2a1ccdfdd23967ad3641ea19afa448
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc7027e0e80cb9bbd62d8d60af57570a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5d1b4afdbe6cc7bd3768b63292b8bc05
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a910d33c239980f3a705e312c5bad6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2eaabc464d6d18a4f362b0452236d65c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8adf1aa119f2fa8c92dc31e5ef07b387 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-172 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate |
2000-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d6d7c52fd41f6c265832be47b1ae03b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82b004c7c5996e1d318e30a8e871f477
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95ec679f3c769a1aff192a188adec78a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1085ad327f4ff8ba0b789d7a1fe837ba |
| publicationDate |
2001-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0121833-A2 |
| titleOfInvention |
Assay for detection of human cftr allele variants using specific diagnostic primers |
| abstract |
A diagnostic method for the detection of the 5T, 7T and 9T alleles in intron (8) of the human CFTR gene which method comprises contacting a test sample of nucleic acid from an individual with a multiplex of diagnostic primers comprising (i) 5T variant primer 5'(N)nAAAGAC3', (ii) 7T variant primer 5'(N*)n*(N)nAAAAGC3' and (iii) 9T variant primer 5'(N*)n*(N)nAAAATC3', wherein N represents additional nucleotides which base pair with the corresponding genomic sequence in the respective allele and n is an integer between 10 and 30 and N* represents additional non-homologous nucleotides which do not base pair with the corresponding genomic sequence in the respective allele and n* is an integer between 5 and 60, in the presence of appropriate nucleotide triphosphates and an agent for polymerisation, such that a diagnostic primer is extended only when the corresponding allelic variant is present in the sample; and detecting the presence or absence of the allelic variant by reference to the presence or absence of a diagnostic primer extension product. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009545314-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008016334-A1 |
| priorityDate |
1999-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |