| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f15bf9f031ed99ff904aba801904f14b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a80de84d6efc1bd0a01c393a5a27c7e4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efbb29fc58e90f99fcdb6bf26d34bd6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5c14d90ca91d8fd4337feec455781c38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_58ab97db9e01a15fccf6f6fc257c4187 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
| filingDate |
2000-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0d70d77cd429773820915f5510a4140
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a6279e8a1cbc467dbe0c9d9647dedcb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05733b477bc420618251473e3c4d972c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db5c5d443293b6a789cb328d3fb88422 |
| publicationDate |
2001-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0120007-A1 |
| titleOfInvention |
A multifunctional system for the efficient manipulation of protein expression in filamentous fungi and method using same |
| abstract |
The present invention relates to expression modules that can be used to assemble integrating and autonomously replicating vectors for use with filamentous fungi. The vectors constructed with these modules are all based on the backbone of the E. Coli plasmid pUC18. They can therefore be used to perform most standard DNA manipulations in the same plasmid that is introduced into the fungus. This system of vectors can be used to study promoter sequences, express heterologous and homologous proteins, and identify vector and host strain mutations that alter protein expression. A subset of these vectors, which include the Aspergillus niger LacA coding region as a reporter gene, were used to develop a high-throughput indicator plate-based screen that could identify host and vector mutants that increase the expression of the secreted reporter protein, LacAp. Finally, the vectors and strains developed can be used to develop strains that can express the coding region for essentially any homologous or heterologous protein at high levels. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006051418-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8017341-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2210899-A1 |
| priorityDate |
1999-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |