| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78587acbfc8d4c9e9ac3baa7021087d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_895f56dec013df46b75536017a756642
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b2df6a72798f7b1a7dec64220016175
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88077763ee540d9ff61ad01aa253c2f1 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
2000-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db2989025c6395610396e0d5fa513315
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23f6723c07978127039f37eadfe6e57c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50e5e242452239f5d903b0907fceaacc |
| publicationDate |
2001-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0109351-A1 |
| titleOfInvention |
Novel vectors and system for selectable targeted integration of transgenes into a chromosome without antibiotic resistance markers |
| abstract |
A method for making a transgenic cell comprising the steps of introducing a linear vector into the cell wherein said vector contains a cassette consisting of sequentially from 5' to 3': a 5' flanking sequence, a polylinker site containing a nucleic acid sequence of interest, and a 3' flanking sequence wherein the 5' and 3' flanking sequences are homologous to a sequence in a chromosome in the cell where the nucleic acid sequence of interest is to be inserted; integration of said vector into the chromosome through recombination between the flanking sequences and the homologous DNA sequences wherein said homologous DNA sequences flank a conditional killing module in a chromosome; and negatively selecting against cells retaining said conditional killing module and deficient for integration of said linear vector. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3399042-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3124613-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9217153-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102482679-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2548809-C2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10487334-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004069996-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004069996-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10752930-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006025702-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7521240-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010135742-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008142028-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7160711-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9683252-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2008252990-B2 |
| priorityDate |
1999-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |