| abstract |
A method and kit for the extraction of DNA from plants is provided, which quickly yields plant DNA with a high level of purity. The method isolates DNA (genomic, chloroplast, and/or mitochondrial DNA) using immobilized anionic groups, preferably on a chromatographic substrate or more preferably magnetic beads derivatized with anionic groups such as diethylaminoethyl (DEAE) via an anion-exchange interaction. The purified DNA is then eluted with ions (typically a salt solution). RNA can be removed by digestion with RNAse. |