| abstract |
The invention provides a method for determining a preferred amino acid sequence motif for an active site an associated substrate specificity subsites of an enzyme. In the method of the invention, an enzyme is contacted with an oriented degenerate peptide library, certain peptides within the library which are substrates for the enzyme are bound by the enzyme, and bound peptide/enzyme complexes are separated from unbound peptides. The bound peptides are released from the enzyme and are sequenced. A preferred amino acid sequence motif for the active site and associated specificity subsites is determined based upon the relative abundance of different amino acids residues at each degenerate position. The invention also provides peptides, peptide analogs, and other small molecules which have a variety of uses and can be derived from the present invention. |