| abstract |
Compositions and methods are provided for identifying factors, including organellar factors, that are differentially expressed when cells in different states, such as metabolic, respiratory, disease or apoptotic states, are compared. In preferred embodiments the invention relates to mitochondria DNA depleted (ς0) and cytoplasmic hybrid (cybrid) cells, such as mitochondrial cybrid cells. Use of the invention to identify species specific expression of organellar factors such as organelle associated macromolecules is contemplated. Also disclosed are examples of organellar factors that are differentially expressed in organelle associated disease, including a variety of human genes that are differentially expressed in Alzheimer's disease. |