| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bfe8e3543d17fddc7ad12fd18b689e09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b5065ba6766a8eb4f1e00e31506e96c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c3899a93c972ce60b070fca823112248 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70589 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5041
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate |
2000-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80f5e51369624a7b98842bb0fb7b5be9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a51b5504e603c978b63216090bebf048 |
| publicationDate |
2000-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0053801-A1 |
| titleOfInvention |
Intact cell assay for protein tyrosine phosphatases |
| abstract |
The invention relates to an intact cell assay for direct quantitation of protein tyrosine phosphatase (PTP) activity using the baculovirus expression system. A PTP expressed in transformed host insect cells is processed and localized in their predicted subcellular compartments. Assays are performed on the PTP expressing host cells challenged with a substrate such as, p-nitrophenyl phosphate. This substrate is hydrolysed to p-nitrophenol by expressed phosphatase activity. Emergence of p-nitrophenol is determined spectrophotometrically and is a measure of PTP activity. Further, the assay and transformed host cells of this invention are particularly useful in a screening method for selecting potential inhibitors to PTPs. Basically, transformed host cells are pre-incubated with or without inhibitors and the assay conducted as described. This invention relates to an intact cell assay with a direct readout of PTP activity that is useful in a cell-based screening method for selecting inhibitors of PTPs. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8658418-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8486636-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8936764-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8298775-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100916651-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8455258-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8273574-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9579650-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10509018-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9643178-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8871446-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10940473-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9926521-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7833708-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9714443-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7887753-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10328428-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10131934-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8420017-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8673645-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9176137-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8163492-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7815868-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009072728-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7820427-B2 |
| priorityDate |
1999-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |