| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d2ec552aaa2423b56a7e226b52fccd4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_761c7bc904ee8fdc41ce084e1d93319d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6564dc8922eecc6b4bbd3de40eed1a0c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5293f8ba9498b4abeba07ecf74b431a2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_482f9d4630331ff060c9a97a5c9d2ee1 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-905
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate |
2000-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_165639f3e85a592ff64bccdd878363ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9d4383356729bffa0f49b0d4d505bdb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdd0b87ff24d4675bf31a7e1c1cf09ed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_609a940f598287095401e0728e17d6bb |
| publicationDate |
2000-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0053781-A1 |
| titleOfInvention |
Generation of conditional yeast mutants, methods and reagents related thereto |
| abstract |
The invention features a method for systematically generating mutant strains of obligate haploid and obligate diploid yeast, having condition-sensitive lethal mutations of a gene which is otherwise essential to cell viability under all conditions of cell growth. Conditional mutants make possible the analysis of physiological changes caused by inactivation of a gene or a gene product and can be used to address the function of any gene. In an exemplary embodiment, the method of the present invention can be generally characterized as follows: (i) providing a Candida strain whichis heterozygous for a loss-of-function mutation of an allele of an essential gene; and (ii) introducing a conditionally sensitive homolog of the gene, e.g., with conditional-sensitive protein or conditional-sensitive transcriptional regulatory sequence, at the functional allele by integrative transformation. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6783985-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02053728-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0160975-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0160975-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-02053728-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1932921-A1 |
| priorityDate |
1999-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |