http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0052200-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b2e3dcd0787092859045990ab8d5d84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8360928fc0313f956e53e751ae1d323 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a78ec6e9064bcbdefee31f3c91ee9fe |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2000-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_999b5edd1276041e1fcf9c9d9f625734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc0b9a9610a20631729e8d1569d322fe |
| publicationDate | 2000-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-0052200-A1 |
| titleOfInvention | METHOD FOR LARGE SCALE cDNA CLONING AND SEQUENCING BY CIRCULATING SUBTRACTION |
| abstract | The present invention provides a method for cDNA sequencing by circulating subtraction, which comprises the steps of: (1) constructing cDNA original libraries with high quality using the tissue of organism as material; (2) according to the grade Cot value assumed, homogenising the cDNA original libraries of step (1) to obtain the cDNA homogeneous libraries; (3) from the cDNA homogeneous libraries of preceding step, choosing clones to sequence DNA; (4) using the cDNA clones which have been sequenced, hybrizing and subtracting the cDNA homogeneous libraries of preceeding step; (5) repeating step (3) and (4) for 1-5000 times. The method can be added a step of preliminary subtractive hybridisation. The method can sequence the cDNA of certain tissue or species effectively and comprehensively. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2004516014-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4856842-B2 |
| priorityDate | 1999-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 197.