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filingDate 2000-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2000-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-0049415-A1
titleOfInvention Chemical and biochemical assay method and apparatus
abstract A method of performing a non-separation assay for determining the level of binding of one component to another. The method comprises the steps of providing a first component in solution. An array of sites is provided onto or into which a second concentrated component is placed. The array of sites is immersed with the solution and scanned with an illuminating light beam such that the light passes through the solution whilst illuminating the sites. The intensity of light received from each of the sites and solution is determined at at least one wavelength during illumination. The received light intensity is used to determine a reference value representative of the solution alone and a value indicative of the amount of binding of the first component to the second component.
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