http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0049172-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c979562c2ac2a114abdebe2fcf69743c |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-922 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
filingDate | 2000-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_104de659b6554ae6d1c7248b453d19b6 |
publicationDate | 2000-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-0049172-A1 |
titleOfInvention | Determination of nuclease activity |
abstract | A method for detecting a nuclease enzyme is disclosed which comprises a method for detecting a nuclease enzyme comprising the steps: a) contacting said enzyme with a compound of formula RpX, wherein R is a 3' nucleosidyl derivative, p is a phospho radical, and X is an esterifiable moiety or, only if R is a 3' nicotinamide derivative, X is an esterifiable moiety or H, whereby ROH and pX are produced, and b) detecting said pX moiety or, only if R is a 3' nicotinamide derivative, detecting the pX moiety or the ROH moiety. In preferred embodiments the invention provides a method for detecting a nuclease enzyme that is free in solution, immobilised on a surface, or attached to a member of a specific binding pair. The method of the invention may thus be applied as a detection step in nucleic acid hybridisation assays, enzyme immunoassays and ligand:receptor binding assays. The invention provides a variety of methods for detecting the detectable moieties produced. These include fluorometric, colorimetric, and luminometric endpoints. Enzyme cycling and apoenzyme reactivation assays are also provided. |
priorityDate | 1999-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 401.