http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0041457-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f11a8255353a71145c081b318e61a149 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02F1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01Q60-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01Q60-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02F1-37 |
| filingDate | 2000-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a603f5a19eb67cf1e9b9bd4c667820a6 |
| publicationDate | 2000-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-0041457-A2 |
| titleOfInvention | Method and device for the optical detection of a particle |
| abstract | The invention relates to a method for detecting individual molecules. The method makes it possible to obtain an extremely good signal-to-noise ratio using non-linear optical effects. A sample support (5) is covered with a layer (4). Said layer has optically non-linear properties, and in particular is suitable for frequency doubling. A molecule of dye (2) is immobilised on the layer (4)and a laser beam (1) is focused onto the molecule through the sample support with limited diffraction. The wavelength of the laser beam corresponds to double the absorption wavelength of the dye molecule. The polarisation of the laser beam is selected according to the strong non-linear susceptibility coefficients of the second order of the optically non-linear layer. In the layer, the frequency of the focused laser light is partially doubled, resulting in a dipolar vibration in the layer at the double frequency. This transfers energy in the near field to the molecule. The emission of the molecule can be detected. The excitation of the dye molecule with the frequency-doubled radiation results in a considerable difference in the wavelengths between the excitation light and the fluorescent emission light. This enables these two signals to be clearly separated and ensures an almost background-free detection of the individual particles. |
| priorityDate | 1999-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.