| abstract |
This invention provides recombinant cells, reaction mixtures, and methods that are useful for the enzymatic synthesis of product saccharides. The recombinant cells contain a heterologous gene that encodes a glycosyltransferase which catalyzes at least one step of the enzymatic synthesis, as well a system for generating a nucleotide sugar that can serve as a substrate for the glycosyltransferase. The recombinant cells, reaction mixtures, and methods are useful for efficiently synthesizing a large variety of saccharides, including polysaccharides, oligosaccharides, glycoproteins and glycolipids, using relatively low-cost starting materials. |