| abstract |
The present invention relates generally to a method for the recombination of at least two nucleic acid sequences and agents useful for same. More particularly, the present invention contemplates a method of recombining, in a host cell which expresses the recBCD nuclease or a functional derivative thereof, a circular nucleic acid sequence with a linear nucleic acid sequence. The method of the present invention is useful, inter alia, for the modification of bacterial artificial chromosomes by homologous recombination with a linear DNA sequence. |