http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0020859-A9
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ef73f36baa533ac8533718a5c9609623 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c4322cb4de342be2dbedcbe0c278f6f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54373 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-648 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 1999-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e950c0f97a9833c17a9a55066ddd54f3 |
| publicationDate | 2000-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-0020859-A9 |
| titleOfInvention | METHOD AND APPARATUS FOR DETECTING BINDING INTERACTIONS $i(IN VIVO) |
| abstract | An apparatus for screening for translocation of a first protein of interest in vivo in a cell comprises (a) a total internal reflection member having a surface portion. If desired, the surface portion can be divided into separate and discrete segments. (b) A cell contacted to the surface portion by the plasma membrane of the cell, the protein having a fluorescent group conjugated thereto. If desired, different cells can be contacted to different ones of the separate and discrete segments. (c) A light source operatively associated with the total internal reflection member and positioned for directing a source light into the member to produce an evanescent field adjacent the surface portion, with the evanescent field extending into a first portion of the cell adjacent the plasma membrane, with the evanescent field being weaker in a second portion of the cell, the fluorescent group emitting light when in the first portion of the cell and emitting less light when in the second portion of the cell (i.e., less light as compared to the amount emitted when the same fluorescent group is in the first portion of the cell). (d) A light detector operatively associated with the total internal reflection member and configured to detect emitted light from the cell. The emission of more or less light from the cell indicates the translocation of the first protein between the first and second portions of the cell. Methods of using the apparatus, particularly for screening for binding or the disruption of binding between first and second proteins of interest, are also disclosed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105283754-A |
| priorityDate | 1998-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 163.