| abstract |
The present invention relates to a method for the preselection of shotgun clones of a genome of an organism, or of parts of the genome of an organism e.g. cosmids, PACs, BACs, etc. that significantly reduces the time and workload associated with the further processing of shotgun clones, for example, in sequencing projects such as the human genome project. The invention relies on a combination of steps including the transfer of shotgun clones to a carrier e.g. nylon membrane, glass chip, etc. where the clones bind, preferably hybridize to a set of specifically selected probes e.g. DNA oligonucleotides, PNA oligonucleotides or pools of DNA or/and PNA oligonucleotides, further antibodies, fragments or derivatives thereof which are labeled or unlabeled. Each probe of said set interacts to 1 to 99 % (ideally 50 %) of all shotgun clones (nucleic acid fragments) in all investigated shotgun libraries. Clones that are characterized as being divergent as a result of the binding experiment in all likelihood represent different parts of the genome or of the investigated part of the genome. The preselection for such divergent clones will reduce the number of redundant analysis of e.g. DNA sequences. |