http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0018881-A8
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_57a631da4914c9e276b8db4b68a9e7d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_675f16e42f230e209d29fb3b0cb54da8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-55 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 1999-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6946bdccdff819b0f1b603007ebedfb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1899dffad6493cf974e8464da89385c1 |
| publicationDate | 2001-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-0018881-A8 |
| titleOfInvention | Intein mediated peptide ligation |
| abstract | An in vitro method for producing a semi-synthetic fusion protein is provided, whereby a target protein fused to an intein - a protein splicing element - is selectively cleaved in a first step as depicted in Figure 1 with a thiol reagent, forming a carboxyl-terminal thioester of the target protein and releasing the target protein from the intein. In a subsequent step as shown in Figure 1, a desired, synthetic, protein or peptide having an amino-terminal cysteine is ligated to the target protein. Standard thiol-reagents such as DTT, or thiol-reagents optimized for ligation such as the odorless MESNA, may be used in the first step. The method permits the direct ligation of a desired peptide to a thioester bond that had linked a target protein to an intein. An in vivo variation of the method will permit production of a cytotoxic protein: a truncated, inactive, form of the protein fused to an intein is introduced in vivo, this fusion product is then selectively cleaved, and a synthetic protein or peptide is subsequently ligated at a carboxyl-terminal thioester of the target protein in order to restore the native activity of the cytotoxic protein. |
| priorityDate | 1998-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.