http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0017219-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9bb6921a45fed2a63edd184b69494052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2a52b50ad880a52274d93c511583354 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0bd8b3b922b2294be79aa009da35a9be |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-705 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 |
filingDate | 1999-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_187a45a463b65139f6a34d036448d9b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cf2cff804aba5e7ef71f1137d052277 |
publicationDate | 2000-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-0017219-A1 |
titleOfInvention | Detection of human herpes virus 6 (hhv6) |
abstract | The present invention relates to methods for detecting viral pathogens, particularly human herpes virus 6 (HHV6), preferably using polymerase chain reaction (PCR) techniques. The present invention also relates to primer sequences useful in these methods. In a first aspect, the present invention consists in an isolated nucleic acid molecule complementary to and specific for human herpes virus 6 (HHV6) DNA including a sequence selected from the group consisting of 5'CTTCTGTTTTAAGTCGTACAGGAGT (SEQ ID NO:1), 5'ACAATTGCCATTTCGGGGAAGTAC (SEQ ID NO:2),and functionally equivalent sequences. A method for detecting HHV6 in a sample suspected of containing HHV6, the method comprising the steps of: (a) optionally amplifying viral DNA present in the sample by polymerase chain reaction techniques using outer primers complementary to the viral DNA; (b) adding to the sample, or to the sample having undergone optional amplification step (a), a pair of inner oligonucleotide primers complementary to and specific for HHV6 DNA, wherein the inner primers comprise the sequences 5'AAGCTTGCACAATGCCAAAAAACAG and 5'CTCGAGTATGCCGAGACCCCTAATC, or functionally equivalent sequences; (c) carrying out polymerase chain reaction techiques on the sample so as to amplify the HHV6 DNA spanned by the inner primers present in the sample; and (d) detecting the amplified HHV6 DNA. |
priorityDate | 1998-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 109.