| abstract |
A method for synthesizing a DNA whereby the time required in the DNA synthesis by the polymerase chain reaction (PCR) method can be shortened, characterized in that PCR is effected under the conditions (A) and (B) as will be defined below with the use of DNA polymerase in an amount effective in giving more than 10 ng of amplified DNA fragments of about 2 kb per 50 νl of a liquid reaction mixture: (A) using 50 νl of a liquid reaction mixture containing DNA polymerase, 1 ng of Escherichia coli genomic DNA and 10 pmol portions of primers Eco-1 and Eco-2 having the base sequences represented respectively by SEQ ID NOS: 1 and 2 in Sequence Listing, and having a composition adequate for the DNA polymerase; and (B) effecting the PCR for 35 cycles with each cycle consisting of 1 second at 99 °C and 7 seconds at 66 °C; a DNA synthesis kit to be used in this DNA synthesis method; and a PCR reagent product. The above method makes it possible to quicken operations in genetic engineering studies and the genetic engineering industry. |