Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a4546b47ff59fa8318a14f80a9a0d071 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1072 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
1999-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7610ef517e481291d50beb8edafb11e9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_674b75ad86b98976fd2d54799cbddeda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4734c73656563cd0b856d39e039d0bf6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c9c962ed13ad1094de08f1ab48fa9f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ccdc13e823a925c772077096ccc2759 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_002b76963d77ccd112bec6947a4ec506 |
publicationDate |
2000-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0009681-A2 |
titleOfInvention |
CONSTRUCTION OF NORMALIZED cDNA LIBRARIES FROM EUCARYOTIC CELLS |
abstract |
A new technology is described that allows for the rapid and efficient construction of complex cDNA libraries from cultured eukaryotic cells. The technology exploits eukaryotic biology by using transgenic constructs that have been nonspecifically inserted into the genome to facilitate the expression of nuclear genes as fusion transcripts. The invention further allows one to specifically subclone the corresponding fusion transcripts into highly complex cDNA libraries. The libraries are easily characterized by molecular analysis techniques such as hybridization, and individual clones can be directly sequenced to generate a sequence database of the cellular portion of the fusion transcripts. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0175127-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0175127-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1279730-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7312075-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8722408-B2 |
priorityDate |
1998-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |