| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6e10d2a9afa49d345275857d8711dc5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_381f84e44beed5559a29e6e54b703e68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37cc05abcc72a26f058e18848ebf03d3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6408
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate |
1999-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08680b66a4d741d52ece0a6449dd3632
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb95abd397cc833ffba6789a7fe96d39 |
| publicationDate |
2000-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0008443-A9 |
| titleOfInvention |
Multiple frequency fluorescence lifetime imaging |
| abstract |
The application of a high frequency periodic bias voltage to the photocathode of an image intensifier device results in a repetitive modulation of the gain characteristics of the intensifier. By controlling the bias or DC component of the bias voltage it is possible to control the duration of the 'on' state of the device relative to its 'off' state. The amplitude of the higher harmonic content in the square waveform can thus be controlled. In this mode the image intensifier can be used as a mixing device for the simultaneous homodyne detection at each of the harmonics. Applied in a fluorescence lifetime imaging microscopy (FLIM) application, fluorescence lifetime images can be obtained at multiple frequencies in a harmonic set calculated from a single series of phase dependent images. Both the phase and modulation parameters necessary for spatially resolved heterogeneous lifetime calculations are extracted by Fourier analysis of the series of phase dependent images. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9268983-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7830575-B2 |
| priorityDate |
1998-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |