http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0001824-A2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c04b5469d2e0e0b4242fb27298eb675c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2fc8c4f23758df7ed658973e2a913e38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97d9eb2515fb15a75aab311a2cf99db9 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2442 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8282 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01014 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 1999-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ea2ee663e8afab84b482c1606cb3f0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19fbb614d8b0b65f91b9740fcbf286aa |
publicationDate | 2000-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-0001824-A2 |
titleOfInvention | Method for genetic engineering of disease resistance using the drr206 class of proteins |
abstract | To identify genes effective against the blackleg fungus Leptosphaeria maculans, we have transformed canola (Brassica napus with four pea (Pisum sativum) genes under constitutive control by the CaMV 35S promoter: PR10.1, chitinase, DRR206 and defensin. Transgenic lines containing single copy T-DNA insertions for each gene were screened for both seedling (cotyledonary) and adult plant resistance. Lines for which pea DRR206 or defensin mRNA was expressed also showed decreased disease scores when inoculated with L. maculans or Sclerotinia sclerotiorum, compared to non-expressing transgenic lines. For PR10 and chitinase transgenics, there was little or no enhancement of resistance. Furthermore, resistance to L. maculans co-segregated with DRR206 and defensin transgenes. Extracts from DRR206 and defensin transgenic plants inhibited fungal growth in-vitro. DRR206 transgenic plants also demonstrated decreased hyphal growth at inoculation sites, and evidence of a hypersensitive response. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1534829-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1534829-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009043182-A1 |
priorityDate | 1998-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 107.