http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE31517-E
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 1978-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1984-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1984-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-RE31517-E |
| titleOfInvention | Method for quantitative determination of renin activity in blood employing phenyl methyl sulfonyl fluoride and polyethylene glycol |
| abstract | A method for the quantitative determination of renin activity in blood utilizing the measurement of Angiotensin I by treating the blood with ethylenediaminetetraacetic acid (EDTA) and the plasma with phenylmethyl sulfonylfluoride (PMSF) at a preferred pH to ascertain the full range of concentration where PMSF is effective for accurate and quick determination. n Also, a method for the quantitative determination of renin activity in blood utilizing the measurement of Angiotensin I by incubating the samples after antibody addition at room temperature (23° to 30° C.) for 1 to 2 hours and separating the free from the antibody bound species with polyethylene glycol after having treated the blood with ethylenediaminetetraacetic acid (EDTA) and the plasma with phenylmethyl sulfonylfluoride (PMSF) at a preferred pH. PMSF offering enough protection against Angiotensin I destruction at room temperature that in combination with polyethylene glycol at a preferred pH the time required for the radioimmunoassay determination is 1 to 2 hours at room temperature instead of 24±2 hours at 4° C. which takes with charcoal in the presence of other inhibitors and making the system less succeptible to time dependent errors. |
| priorityDate | 1973-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.