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filingDate 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9994840-B2
titleOfInvention Method and apparatus for extracting DNA from a biological sample
abstract A method of extracting DNA from a biological sample includes the steps of freezing a biological sample; mechanically breaking down the sample to produce a processed sample; mixing the processed sample with a lysis solution; and cycling the mixture through a series of pressure fluctuations to produce a resulting lysate. The method may further include the steps of exposing the lysate to a material that binds to DNA present in the sample and collecting the DNA from the sample using an elution solution. An apparatus for practicing the method includes a first segment for mechanically breaking down the sample; a second segment for pressurizing a mixture of the processed sample and lysis solution to produce a lysate; and wherein the first segment is fluidly coupled to the second segment. The apparatus may also include a third segment in which the lysate is exposed to the binding material.
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