Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0506c17f5fb143b8d86345300758dade http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07cb693e4064ab67d08fd10f30ceaabc |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-117 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate |
2014-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6be42c245b1a10cfd75334d3e742d8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7577c9a0a28f753a8e85f2373c0629f |
publicationDate |
2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9988659-B1 |
titleOfInvention |
In vivo conversion of nucleosides in plasmid DNA |
abstract |
The instant invention provides for the assembly of large DNA oligonucleotide constructs by the self-assembly of multiple oligonucleotide fragments, wherein the assembly is guided by the hybridization between non-standard nucleotides that form non-standard nucleobase pairs orthogonal to the standard T:A and C:G nucleobase pairs. Adding nucleobase pairs increases the information density of the fragments, minimizing off-target hybridization. The invention further provides rules and methods for converting non-standard pairs into standard pairs using polymerase copying with conversion. |
priorityDate |
2009-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |